Studies on the Ribonuclease in Posterior Silkglands of Silkworm Larvae, Bombyx mori. : 2. Specincity of the Ribonuclease

家蚕後部絹糸腺のリボヌクレアーゼに関する研究 : 第Ⅱ報酵素の特異性

Studies on the Ribonuclease in Posterior Silkglands of Silkworm Larvae, Bombyx mori. : 2. Specincity of the Ribonuclease

Creators Numa Sinji

Creators Mukai Jun-Itiro

Creators Akune Satoru

One of the two ribonucleases detected in posterior silkglands of silkworm was purified by using of ammonium sulfate fractionation, dialysis, carboxymethyl cellulose column chromatography and Sephadex G-100 gel filtration. The specificity was determined on the completely and incompletely digested yeast RNA by paper chromatography and column chromatography. This enzyme splits C'_5-phosphate bond of 3', 5'-phosphodiester linkages of ribonucleic acid. 2'-and 3'-Adenylic acid, guanylic acid, 3'-(and, or 2'-) cytidylie acid and uridylic acid (perhaps 3'-and, or 2'-) were formed, with cyclic mononucleotides as intermediate priduct.

[ISSN]0513-1715

[NCID]AN00244250