Studies of placental alkaline phosphatase by means of lectin affinity electrophoresis

レクチン親和電気泳動法を用いた胎盤性ALPの研究

Studies of placental alkaline phosphatase by means of lectin affinity electrophoresis

Creators Tomimatsu Takeshi

1) The author has developed a new type of lectin affinity electraphoresis (AEP) using polyacrylamide-gel (PAG) with Lentil-lectin (LCH) as the affinity ligand. By this method, placental alkaline phosphatase was separated into 4 distinct bands (PLALP I ～IV subgroups) with different affinity for LCH. 2) According to affinity constants theory, the dissociation constants of PLAP-LCH interaction were determined. The obtained dissociation constants revealed that PLALP I has the strongest affinity for LCH and PLALP IV has the weakest affinity. 3) The author has also developed a two dimensional affinity electrophoresis (1st. Dimention : isoelectric focusing, 2nd. Dimention : lectin AEP). By this method, each PLALP subgroup was further separated into 5～6 fractions having different isoelectric point. The origin of the PLALP polymorphism in AEP was based on the affinity of the enzymes for LCH.

[ISSN]0513-1731

[NCID]AN00243156