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Studies on the Inactivation Test of Japanese Encephalitis Vaccine : Proposal of a New Method Using Tissue Culture Cells

山口医学 Volume 33 Issue 3 Page 105-120
published_at 1984-06
Title
日本脳炎ワクチンの不活化試験法に関する研究 : 組織培養細胞を使用した生残ウイルス否定試験法
Studies on the Inactivation Test of Japanese Encephalitis Vaccine : Proposal of a New Method Using Tissue Culture Cells
Creators Kanno Masayoshi
Creators Yoshii Zensaku
Creators Kobayashi Susumu
Creators Yamamoto Shigeo
Creators Yamashita Koshi
Creators Tsunehisa Yoshinao
Creators Matsuyama Shigeo
Source Identifiers
An improved method for the inactivation test of Japanese encephalitis vaccine is proposed. The principle lies in the detection of residual viruses by inoculating large amount of test samples into the tissue culture cells. The process consists of 3 parts. 1) The introduction of primary cell cultures of hamster kidney. 2) Removal of cytotoxic substances -formalin and thimerosal- from test samples by thorough dialysis. 3) Long period (4 weeks) observations of the primary inoculation bottles and two parallel passages for the appearance of cytopathic effects. The results covered the recovery of routine method and its efficiency is higher than that of the legally determined method of using intracerebral inoculation of mice.
Subjects
医学 ( Other)
Languages jpn
Resource Type journal article
Publishers 山口大学医学会
Date Issued 1984-06
File Version Not Applicable (or Unknown)
Access Rights metadata only access
Relations
[ISSN]0513-1731
[NCID]AN00243156
Schools 医学部