Identification of Abnormal Hemoglobins IV. Polyacrylamide Gel Electrophoresis of the Globin Chains

異常血色素の同定法 IV グロビン鎖のポリアクリルアミドゲル電気泳動

Identification of Abnormal Hemoglobins IV. Polyacrylamide Gel Electrophoresis of the Globin Chains

Creators Fujisawa Keiko

Creators Muroya Satomi

Creators Minooka Hiromi

Creators Ohba Yuzo

Polyacrylamide gel electrophoresis of the globin chains using two buffer systems, tris-glycine (pH9.8) and β-alanne acetate (pH4.6) in concentrated urea, was studied by column and slab methods. Concentration of urea was reduced from 8 M to 6.4 M for convenience without change in resolving power. In order to save time, total acrylamide concentration of 4.9g/dl was preferred to 7.3g/dl. The pH 9.8 buffer was quite suitable for the separation of the α,β and σ chains, while the pH 4.6 buffer was particularly useful for the separation of the γ from the βchain and of the N-acetyland γ chain from the unmodified one…

[ISSN]0513-1731

[NCID]AN00243156