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Superoxide stimulation enhances CXCR4 expression in heart muscle-derived stem cells via ASK1 activation

The bulletin of the Yamaguchi Medical School Volume 60 Issue 1-2 Page 11-18
published_at 2013
A050060000102.pdf
[fulltext] 1.06 MB
Title
Superoxide stimulation enhances CXCR4 expression in heart muscle-derived stem cells via ASK1 activation
Creators Akashi Shintaro
Creators Miura Toshiro
Creators Shibuya Masaki
Creators Fukagawa Yasuhiro
Creators Oda Takamasa
Creators Nakamura Takeshi
Creators Ikeda Yasuhiro
Creators Matsuzaki Masunori
Creators Yano Masafumi
Source Identifiers
Stem cell therapy for cardiac regeneration is hindered by poor homing to infarcted legion because of poor expression of homing factor, CXCR4, on the stem cell surface. A strategy for enhancing CXCR4 is promising to acceler- ate stem cell homing to infarcted lesion. Methods and Results: Heart muscle-derived Sca-1(+) cells (HDSCs) were isolated from normal mouse heart using surface antigen Sca-1. The expression of CXCR4 was determined by the fluorescence-activated cell sorting by labeling the cells with CXCR4 antibody with fluorescence. The expression of CXCR4 was enhanced by stimulation of hydrogen peroxide (0.1, 0.33, 1μM) for 24h. The Western blotting showed no significant increase in the protein level of CXCR4. The enhancement of CXCR4 can be attributed to translocation of CXCR4 from the cytosolic fraction to cell surface. H_2O_2 stimulation enhanced ASK1 p966 phosphory- lation. Conclusions: The expression of CXCR4 on HDSCs was augmented by H_2O_2 through the translocation from cytosolic to membrane surface through ASK1 phos-phorylation
Languages eng
Resource Type departmental bulletin paper
Publishers Yamaguchi University School of Medicine
Date Issued 2013
File Version Version of Record
Access Rights open access
Relations
[ISSN]0513-1812
[NCID]AA00594272
Schools 医学部附属病院 大学院医学系研究科(医学)