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Role of alpha-actinin-4 in the regulation of endocytosis

The bulletin of the Yamaguchi Medical School Volume 55 Issue 3-4 Page 21-26
published_at 2008
A050055000301.pdf
[fulltext] 571 KB
Title
Role of alpha-actinin-4 in the regulation of endocytosis
Creators Hara Tomohiko
Creators Honda Kazufumi
Creators Shitashige Miki
Creators Ono Masaya
Creators Matsuyama Hideyasu
Creators Naito Katsusuke
Creators Hirohashi Setsuo
Creators Yamada Tesshi
Source Identifiers
Creator Keywords
actinin-4 endocytosis clathrin proteomics
Alpha-actinin-4, originally identified as an actin-binding protein, has been associated with cell motility and cancer invasion. However, it forms complexes with a diverse array of partner proteins and is speculated to exert several distinct functions based on the characteristics of these proteins. Immunoprecipitation and mass spectrometric analysis reveal that alpha-actinin-4 forms native complexes with several partner proteins in 22RV1 cells, including β/γ-actin, clathrin heavy chain and nonmuscular myosin heavy chain. Clathrin is a coat protein that covers the internalized membrane pit and plays a central role in early endocytosis. We identified other clathrin-related and unrelated cargo proteins, including dynamin, adaptin-γ, β-NAP and p47A, that interact with alpha-actinin-4. Immunofluorescence microscopy revealed that dynamin and clathrin are co-localized with alpha-actinin-4 at the dorsal sites of membrane rufflings, and the transfection of ACTN4 cDNA facilitates the transport of transferrin into the peri-nuclear endosomes. In this review, we report that alpha-actinin-4 is involved in the regulation of endocytosis. Endocytosis enforces termination of signalling evoked by the cell surface receptors and regulates recycling of receptors and ligands. The change of alpha-actinin-4 expression levels may therefore induce aberrations in the intracellular trafficking of various cell surface molecules, such as growth factors and receptors.
Subjects
医学 ( Other)
Languages eng
Resource Type departmental bulletin paper
Publishers Yamaguchi University School of Medicine
Date Issued 2008
File Version Version of Record
Access Rights open access
Relations
[isVersionOf] [URI]http://ds.cc.yamaguchi-u.ac.jp/~bulletin/index.html
Schools 大学院医学系研究科(医学)