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A Sensitive Method for Detecting Sudunit Dissociation of Abnormal Hemoglabin

The bulletin of the Yamaguchi Medical School Volume 30 Issue 1 Page 11-19
published_at 1983-12
A020030000102.pdf
[fulltext] 539 KB
Title
A Sensitive Method for Detecting Sudunit Dissociation of Abnormal Hemoglabin
Creators Hattori Yukio
Creators Ohba Yuzo
Creators Miyaji Takaoki
Source Identifiers
Creator Keywords
Abnormal hemoglobin Subunit dissociation
Dissociation of abnormal hemoglobin (HbA) by gel permeation employing Sephadex G-75 column. Equimolar solutions(5чM)of Hb A and an abnolmal hemoglobin in 0.01 M phosphate buffer, 7.00 containing 0.09 M NaCl and a trace amount of KCM, are introduced into the column (1.0×60 cm,upward flow) by a peristatic pump (flow rate 0.25 ml/min) one after another. The absorbance at 280 nm or Soret band of the effluent is continuously monitored. The difference in the degree of dissociation is detected by the change in the absorbance at the interface where the two hemoglobins meet. The method developed here has been applied to fifteen different abnormal hemoglobins; twelve of them (80%) have shown either accelerated or reduced dissociation. The method is highly sensitive and specific for the detection of abnormal hemoglobins.
Subjects
医学 ( Other)
Languages eng
Resource Type departmental bulletin paper
Publishers Yamaguchi University School of Medicine
Date Issued 1983-12
File Version Version of Record
Access Rights open access
Relations
[ISSN]0513-1812
[NCID]AA00594272
Schools 医学部