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Antigenic identification of excretory-secretory products of adult Dirofilaria immitis

Japanese journal of veterinary science Volume 52 Issue 5 Page 995-1000
published_at 1990
2008010280.pdf
[fulltext] 977 KB
Title
Antigenic identification of excretory-secretory products of adult Dirofilaria immitis
Creators Kaneko Hideto
Creators Hayasaki Mineo
Creators Ohishi Isamu
Creator Keywords
Dirofilaria immitis dog excretory-secretory product immunoblotting
Excretory-secretory (ES) products collected from adult Dirofilaria immitis cultured in vitro were analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting. ES products of male (M-ES) and female (F-ES) worms were separated into 16 and 21bands by SDS-PAGE with Coomassie blue and silver staining, respectively. The antigenic bands were then analyzed by immunoblotting, using pooled sera from dogs that had naturally contracted D. immitis. Sera from dogs with microfilaremic infection showed 7 bands in M-ES and 10 bands in F-ES, while those from dogs with occult infection revealed 3 bands in M-ES and 10 bands in F-ES. Among these bands, those of 14, 18, 21, 22, 29, and 32 kilodaltons (Kd) were common tho M-ES and F-ES, those of 39 and 44 Kd were specific to M-ES, and those of 20, 38, 43, 53, 63, 90, 110, 125 and 136 Kd were specific to F-ES.
Languages eng
Resource Type journal article
Publishers 日本獣医学会
Date Issued 1990
File Version Version of Record
Access Rights open access
Relations
[ISSN]0021-5295
[NCID]AA10521639
[isVersionOf] [URI]http://www.jstage.jst.go.jp/browse/jvms/-char/ja
[isVersionOf] Dirofilaria immitis infection and dirofilariosis : an animal model of human filariosis [URI]http://www.lib.yamaguchi-u.ac.jp/yunoca/handle/2010010511
Schools 農学部